Department of Bioscience Technology
College of Science - Department of Bioscience Technology
Ministry of Education assented to plan and set up the Departmant of Bioscience Technology of Chung Yuan Christian University in 2000. Ministry of Education approved to establish the Departmant of Bioscience Technology of Chung Yuan Christian University in 2001. Departmant of Bioscience Technology was established in 2002 and recruited the first undergradurate students.
Currently, our department has undergraduate and master course. Our undergraduate course has 50 students per year since 2002, however, we extend undergraduate course up 100 students from year 2012. From 2007, our master course recruits 14 students to improve our research. So far, our master course have 18 students per year.
The goals of our department described as below.
- To educate our student to have strong background of Biotechnology including technology and theory.
- To train our student to have excellent ability of experiments of life science.
- To incubate our student to have independently research and innovation skills that are niche for jobs.
- To provide fundamental Biological course for other department students to teach interdisciplinary students.
- Our master courses match the Biotechnology policy of our country to instruct the specialist of Bio-medicine and health food technology.
In order to cooperate with the national development in science and technology, the Executive Organ of ROC classified Biotechnology as the eight key science and technology in 1982, and prepared to establish the research and development unit of Biotechnology at the same time. The Executive Organ of ROC passed the scheme for "strengthening the Biotechnological industry and promote" and urged Ministry o Education to be responsible for talents` cultivation and improvement of the Biosecience education in 1995. This is an important subject for our department to combine the Biotechnological relevant courses, raise course quality, train relevant technology and familiar with relevant regulation
We designed our courses related to theory and practices. In undergraduate course, we designed essential and elective courses including fundamental, core, liberal and specialty, in addition, seminar and topic practice related courses will train students for individually research. For postgraduate course, we arrange the core course (using “The Cell” text book) which is divided into four sections as 8 credits to support the basic knowledge. We also provide different topic courses up to 14 credits for Bio-medicine, neurology, embryology, oncology, Biotechnology, food toxicology and food science. In addition, our also arrange the training course for instrument and animal caring. Three major programs, life science, Bioinformatics, and food science, are hold for students to take re-organized courses. The regular academic activities of our department are
1) to invite the visiting professor to support our education and research,
2) to hold research compete,
3) to encourage our students to join academic conference,
4) to visit Bio-tech and food company,
5) To launch the industry cooperation project to provide multiple training channels for students.
We focus our research topics are
1) the development of stem cells,
2) Bioscience health foods,
3) multiple expression system,
4) Bioinformatics. For education topics, we focus on Bio-medicine and health science for our department characteristics.
The developing direction and common characteristic of Chung Yuan Christian University will combine "Biotechnology" with "the whole people educate". This school had already established " Biotechnology and project promotion committee.This school had already promoted actively and set about building the "Biotechnology and Project Building". The developing direction of Department of Bioscience Technology divide stating as follows especially:
VIA Technologies, Inc. (VIA) offers and establishes "VIA cord blood Stem Foundation" and already sign contracts with this school. VIA cord blood Stem Foundation had already garrisonned in our department and purchased more than one hundred million N.T. dollars to buy the latest instruments for research. So the teachers and graduate students of this department can participate in the research works in this foundation directly. Many professors had engaged in embryo or stem cell research already in our department. So stem cell based on research in our department is the most characteristic among the domestic universities.
Traditional Chinese Medicine
Do you know that the good health food now needn`t go to the remote mountains to adopt the wild one again? Through the choice and decorating by Biotechnology, we can produce better and more health food. The health food has practical and potentiality most in the Biological development in science and technology at the present stage. Besides, health food is a key development in our country. In addition, this department have many professors of leading position in nutrition and food science circles. We carry on clinical research and cooperate with Tao-Yuan General Hospital Department of Health.
A lot of unknowable DNA sequences in research are expressed by Bioinformatics and became meaningful information. Bioinformatics develops the most scarce needing talents of Biotechnology for the whole world at present. Chung Yuan Christian University have strongest Department of Information and Computer Engineering, Business Administration and Mechanical Engineering, the teachers who work for similar specialities and backgrounds in mathematics, chemistry or chemical engineering.So Chung Yuan Christian University should have potentiality to develop Bioinformatics.
The future prospects for our research are:
Dr. Ming-Wei Chao
Dr. Chao was investigating mechanisms responsible for the induction of cytotoxicity and GPT/APRT mutagenicity in nuclear excision repair (NER) deficient CHO cells. To date he has extensively characterized DNA damage, mutagenesis, and the production of ROS in this system. His pre-doctoral and postdoctoral training and experience was in an outstanding laboratory, in which he employed experimental approaches of direct relevance to his current work.
Dr. Guang-Jer Wu
This laboratory focuses on the molecular Biology of metastasis of prostate cancer, ovarian cancer, breast cancer, melanoma, and nasopharyngeal carcinoma. Over-expression of a cell adhesion molecule, METCAM/MUC18 (CD146, MUC18), has been correlated with the metastatic propensity of melanoma cells. To detect the expression of METCAM/MUC18 in different human cancer cell lines and tissues, we have cloned the human and mouse METCAM/MUC18 (huMETCAM/MUC18 and moMETCAM/MUC18) cDNA genes and over-expressed and purified the recombinant proteins for the preparation of polyclonal antibodies in chicken.
Dr Wen-Hsiung Chan
Emodin (1,3,8-trihydroxy-6-methylanthraquinone), a major constituent of rhubarb, has a wide range of therapeutic applications. Previous studies have established that emodin induces apoptosis in the inner cell mass and trophectoderm of mouse blastocysts and leads to decreased embryonic development and viability, indicating a role as an injury risk factor for normal embryonic development. However, the mechanisms underlying its hazardous effects have yet to be characterized. In the current study, we further investigated the effects of emodin on oocyte maturation and subsequent pre- and post-implantation development, both in vitro and in vivo. Notably, emodin induced a significant reduction in the rates of oocyte maturation, fertilization, and in vitro embryonic development. Treatment of oocytes with emodin during in vitro maturation (IVM) led to increased resorption of postimplantation embryos and decreased fetal weight. Experiments using an in vivo mouse model disclosed that consumption of drinking water containing 20–40 μM emodin led to decreased oocyte maturation and in vitro fertilization, as well as early embryonic developmental injury. Notably, pretreatment with a caspase-3-specific inhibitor effectively prevented emodin-triggered injury effects, suggesting that impairment of embryo development occurs via a caspase-dependent apoptotic process.
Dr Ivy Hsu
Oral cancer has becomes the most prominent cancer disease in recent years in Taiwan. The reason is the betel nut chewing habit combing with smoking and alcohol-drinking lifestyle of people results in oral cancer becomes the fastest growth incident cancer amongst other major cancer diseases. In previous studies showed that photosan, haematoporphyrin derivative (HPD), has demonstrated effective PDT results on human head and neck disease studies. To avoid the systemic phototoxic effect of photosan, this study was designed to use a topical photosan-mediated PDT for treatment of DMBA-induced hamster buccal pouch cancerous lesions. DMBA was applied to one of the buccal pouches of hamsters thrice a week for 10 to 12 weeks. Cancerous lesions were induced and proven by histological examination. These DMBA-induced cancerous lesions were used for testing the efficacy of topical photosan-mediated PDT. Before PDT, fluorescence spectroscopy was used to determine when photosan reached its peak level in the lesional epithelial cells after topical application of photosan gel. We found that photosan reached its peak level in cancerous lesions about 13.5 min after topical application of photosan gel. The cancerous lesions in hamsters were then treated with topical photosan-mediated PDT (fluence rate: 600 mW/cm2; light exposure dose 200 J/cm2) using the portable Lumacare 635 nm fiber-guided light device. Visual examination demonstrated that topical photosan-mediated PDT was an applicable treatment modality for DMBA-induced hamster buccal pouch cancerous lesions.
Dr Ting-Yu Chin To clarify the involvement of autophagy in neuronal differentiation, the effect of rapamycin, an mTOR complex inhibitor, on the dibutyryl cAMP (dbcAMP)-induced differentiation of NG108-15 cells was examined. Treatment of NG108-15 cells with 1 mM dbcAMP resulted in induction of differentiation, including neurite outgrowth and varicosity formation, enhanced voltage-sensitive Ca2+ channel activity and expression of microtubule-associated protein 2, and these effects involved phosphorylation of cAMP-response element binding protein (CREB) and extracellular signal regulated kinase (ERK). Simultaneous application of dbcAMP and rapamycin synergistically increased and accelerated differentiation. mTOR or raptor silencing with siRNA had a similar effect to rapamycin. Rapamycin and silencing of mTOR or raptor evoked autophagy, while blockade of autophagy by addition of 3-methyladenine or beclin 1 or Atg5 silencing prevented the potentiation of differentiation. Silencing of rictor also evokes autophagy, at a level 55% of that induced by raptor silencing and enhancement of differentiation is proportional. Rapamycin also caused increased ATP generation and cell cycle arrest in G0/G1 phase, but had no effect on CREB and ERK phosphorylation. dbcAMP also induced ATP generation, but not autophagy or cell cycle arrest. These results suggest that the increased autophagy, ATP generation and cell cycle arrest caused by mTOR inhibition promotes the dbcAMP-induced differentiation of NG108-15 cells.
Dr Chung-Der Hsiao Zebrafish skin is composed of enveloping and basal layers which form a first-line defense system against pathogens. Zebrafish epidermis contains ionocytes and mucous cells that aid secretion of acid/ions or mucous through skin. The killer/testing line binary system established in the current study demonstrates a nitroreductase/metrodinazole system that can be utilized to conditionally perform skin ablation in a real-time manner, and provides a valuable tool to visualize and quantify the anti-apoptotic potential of interesting target genes in vivo. The current work identifies a potential use for transgenic zebrafish as a high-throughput platform to validate potential apoptosis modulators in vivo.
Dr Shu-Rung Lin The liver architecture plays an important role in maintaining hemodynamic balance, but the mechanisms that underlie this role are not fully understood. Hepsin, a type II transmembrane serine protease, is predominantly expressed in the liver, but has no known physiological functions. Here, we report that hemodynamic balance in the liver is regulated through hepsin. Deletion of hepsin (hepsin−/−) in mice resulted in enlarged hepatocytes and narrowed liver sinusoids. Using fluorescent microbeads and antihepsin treatment, we demonstrated that metastatic cancer cells preferentially colonized the hepsin−/− mouse liver as a result of the retention of tumor cells because of narrower sinusoids. The enlarged hepatocytes expressed increased levels of connexin, which resulted from defective prohepatocyte growth factor (pro-HGF) processing and decreased c-Met phosphorylation in the livers of hepsin−/− mice. Treatment of hepsin−/− mice with recombinant HGF rescued these phenotypes, and treatment of wild-type mice with an HGF antagonist recapitulated the phenotypes observed in hepsin−/− mice. Conclusion: Our findings show that the maintenance of hepatic structural homeostasis occurs through HGF/c-Met/connexin signaling by hepsin, and hepsin-mediated changes in liver architecture significantly enhance tumor metastasis to the liver.
Dr Liang-Yi Wu Insulin and (−)-epigallocatechin gallate (EGCG) are reported to regulate obesity and fat accumulation, respectively. This study investigated the pathways involved in EGCG modulation of insulin-stimulated glucose uptake in 3T3-L1 and C3H10T1/2 adipocytes. EGCG inhibited insulin stimulation of adipocyte glucose uptake in a dose- and time-dependent manner. The concentration of EGCG that decreased insulin-stimulated glucose uptake by 50-60 % was approximately 5-10 µM for a period of 2 h. At 10 µM, EGCG and gallic acid were more effective than (−)-epicatechin, (−)-epigallocatechin, and (−)-epicatechin 3-gallate. We identified the EGCG receptor [also known as the 67-kDa laminin receptor (67LR)] in fat cells and extended the findings for this study to clarify whether EGCG-induced changes in insulin-stimulated glucose uptake in adipocytes could be mediated through the 67LR. Pretreatment of adipocytes with a 67LR antibody, but not normal rabbit immunoglobulin, prevented the effects of EGCG on insulin-increased glucose uptake. This suggests that the 67LR mediates the effect of EGCG on insulin-stimulated glucose uptake in adipocytes. Moreover, pretreatment with an AMP-activated protein kinase (AMPK) inhibitor, such as compound C, but not with a glutathione (GSH) activator, such as N-acetyl-L-cysteine (NAC), blocked the antiinsulin effect of EGCG on adipocyte glucose uptake. These data suggest that EGCG exerts its anti-insulin action on adipocyte glucose uptake via the AMPK, but not the GSH, pathway. The results of this study possibly support that EGCG mediates fat content.
Dr Tzong-Yuan Wu Host protein synthesis is shut down in the lytic baculovirus expression vector system (BEVS). This also affects host proteins involved in routing secretory proteins through the endoplasmic reticulum (ER)-Golgi system. It has been demonstrated that a secretory alkaline phosphatase–EGFP fusion protein (SEFP) can act as a traceable and sensitive secretory reporter protein in BEVS. In this study, a chaperone, calreticulin (CALR), and the translation initiation factor eIF4E were co-expressed with SEFP using a bicistronic baculovirus expression vector. We observed that the intracellular distribution of SEFP in cells co-expressing CALR was different from co-expressing eIF4E. The increased green fluorescence emitted by cells co-expressing CALR had a good correlation with the abundance of intracellular SEFP protein and an unconventional ER expansion. Cells co-expressing eIF4E, on the other hand, showed an increase in extracellular SEAP activity compared to the control. Utilization of these baculovirus expression constructs containing either eIF4E or CALR offers a significant advantage for producing secreted proteins for various Biotechnological and therapeutic applications.
Dr Chung-Yung Chen Bone marrow hematopoietic stem cells (HSCs) are crucial to maintain lifelong production of all blood cells. HSCs divide can differentiate into many kinds of blood cell types such as T cells, B cells and macrophages. HSCs originate during a differentiation period of active epigenetic remodeling. DNA methylation patterns are likely to be critical for their self-renewal, development and differentiation. Aberrant methylation of CpG islands is associated with transcriptional inactivation of genes involved in cell development, such as cell-cycle control, apoptosis signalling and DNA repair. Clinical trials of epigenetic therapies are now in progress, and epigenetic profiling using DNA methylation will provide guidance on optimization of the use of these therapies with conventional chemotherapy, as well as helping to identify patient populations who may particularly benefit from such approaches. To understand epigenetic patterns of differentiation, the PCR selective suppression hybridization strategy was designed to fetch all of genes that were changed their methylation statuses in CpG islands. After analysis of HSCs and CD8+ T cells using this approach, hundreds of methylated genes were recovered during comparison of HSCs and CD8+ T cells. Thus, this technology is able to detect methylation of CpG islands as high sensitivity and specificity and help to reveal the epigenetic CpG island evens during differentiation.
Bachelor of Science
The basic requirements for a Bachelor of Science in Bioscience Technology specify that a student needs to finish the curriculum of 128 semester hours which includes the 88 semester credits of required and 40 credits of elective courses. Our undergraduate course has 50 students per year since 2002, however, we extend undergraduate course up 100 students from year 2012. We are welcome any foreign students to joint our undergraduate course.
Three major programs, life science, Bioinformatics, and food science, are hold for students to take. Our future plans for education and research focus on “Biomedicine technology” and “Health food technology”. We educate our students for those two specialties. However, we also educate our students to learn Bio-technology knowledge and particle operation skills. We lunch “cooperated project” to promote “Biomedicine technology” and “Health food technology” researches.
To achieve our aims, the undergraduate courses are arranged to match those two goals. For fundamental course, students are required to take “Computer”, “Chemistry”, “Organic Chemistry”, “Analytical Chemistry” and “Calculus”. For core courses, students are required to take “Biology”. “Biochemistry”, “MicroBiology” and “Human Physiology”. For specialty courses, students are required to take “Cell Biology”, “Molecular Biology”, “Stem Cells”, “Metabolic Biochemistry”, “Bioinformatics”, “Health Food” and “Nutrition”.
We encourage our students to take other department’s courses or programs to train for other skill and knowledge. For “General Education”, students are required to take four different catalog courses including “Universal”, “Character”, “Physical” and “Personality”.
Master of Science
A minimum of 30 graduate credits is required. Of these 30 credits, includes the 8 semester credits of required, 16 credits of elective courses and 6 credits of the thesis. A final oral examination is also required.
Every master students require to be signed a supervisor to moniter the master research and thesis. The research topics of our faculty described as below:
Dr. Guang-Jer Wu
Major research topics are molecular mechanisms of Prostate Cancer,Ovarian Cancer,Breast Cancer,Melanoma and Nasopharyngeal. The Specialties of teaching and research are cancer Biochemistry and molecular Biology.
Dr Tzong-Yuan Wu
Major research topics are developments of Baculoviru expression system and application for Biological pesticide. The Specialties of teaching and research are Biochemistry and molecular Biology.
Dr Wen-Hsiung Chan
Major research topics are molecular mechanisms of apoptosis and embryonic development. The Specialties of teaching and research are Biochemistry, signal transduction and protein.
Dr Ivy Hsu
Major research topics are personal medicine. The Specialties of teaching and research are nutrition, photodynamic therapy and Biomedicine management.
Dr Chung-Yung Chen
Major research topics are stem cells, epigenomics, microbe genomics and Bioinformatics. The Specialties of teaching and research are genetics and Bioinformatics.
Dr Shu-Rung Lin
Major research topics are hemophilia genetic identification, gene knot-out mice and related physiological functions and pathological analysis. The Specialties of teaching and research are gene knot-out, trans-gene animal, molecular Biology and hematology.
Dr Ting-Yu Chin
Major research topics are differentiation mechanisms of neural stem cells. The Specialties of teaching and research are cell Biology, signal transduction.
Dr Liang-Yi WuMajor research topics are functional evaluation of health food and pathogenesis of metabolism syndrome. The Specialties of teaching and research are metabolic Biochemistry and food and nutrition.
Dr Chung-Der Hsiao Major research topics are signal regulation of epidermal stem cells and trans-gene zebra fishes. The Specialties of teaching and research are embryology and genomics.
Dr. Ming-Wei Chao Major research topics are DNA mutation by toxic compound and health food for prevention of cardiovascular. The Specialties of teaching and research are pharmacology, toxicology and free radical Biology.
Dr Yi-Ming Chen Major research topics are immune response by microbial and related diagnosis and vaccine development. The Specialties of teaching and research are microBiology and immunology.